Oi:ten.1371/journal.pone.0061905.ga-Amanitin TreatmentRNA polymerase II was inhibited by adding
Oi:10.1371/journal.pone.0061905.ga-Amanitin TreatmentRNA polymerase II was inhibited by adding 10 mg/ml aamanitin (Sigma) for the duration of the BrU labeling step described above.Ribonuclease TreatmentAfter the wash step to eliminate soluble BrU,…